摘要
Abstract
TABLE OF CONTENTS
List of Abbreviations
CHAPTER 1：Introduction
1.1 Rice importance and its History
1.2Genes responsible for grain size，width and thousand grain weight
1.2.1 GS2(GRAIN SIZE 2)
1.2.2GS3(GRAIN SIZE 3)
1.2.4 GS6(GRAIN SIZE 6)
1.2.6 GW2(GRAIN WIDTH 2)
1.2.8 GW7/GL7(GRAIN WIDTH 7/GRAIN LENGTH 7)
1.2.10 GW8(GRAIN WIDTH 8)
1.2.12 TGW6(THOUSAND-GRAIN WEIGHT 6)
1.3 Insertion/Deletion (InDel) markers
1.4 Importance and morphology of Flag leaf length in rice
1.4.1 Quantitative trait loci (QTLs) and fine mapping for flag leaf length (FLL) in rice
1.5 Importance and morphology of panicle length (PL) in rice
1.5.1 Quantitative trait loci (QTLs) and fine mapping for panicle length (PL) in rice
1.6 Quantitative trait loci (QTLs) mapping and Fine-mapping
1.7 Candidate Genes Analysis
CHAPTER 2: InDel marker-based estimation of multi-gene allele contribution and genetic variations for grain size and weight in rice (Otyza sativa L.)
2.1Introduction
2.2Materials and methods
2.2.3 Marker Genotyping
2.2.4 Statistical analyses
2.3Results
2.3.1 Descriptive statistics and phenotypic variability for rice grain size and weight
2.3.2 Factor analysis and phenotypic correlation among four traits for rice grain size and weight
2.3.3 InDel based estimation of allelic distribution of genes in determination of grain size and weight
2.3.4 Favorable alleles of studied genes for gene pyramiding
2.3.5 InDel Polymorphism and assessment of genetic relationship among 204 rice germplasm
2.3.6 Estimation of population genetics for grain size and weight based on fourteen InDel markers
2.4Discussions
2.5Conclusion
CHAPTER 3: Fine mapping of the QTL qLFP9 controlling flag leaf and panicle length in rice (Otyza sativa L.)
3.1 Introduction
3.2Material and methods
3.2.1Plant materials
3.2.2Measurement of agronomic traits
3.2.3DNA extraction
3.2.4Polymerase chain reaction(PCR) and electrophoresis analysis
3.2.4Gel electrophoresis analysis
3.2.6 RNA extraction and RT-PCR reaction
3.2.7Chi-square test
3.2.8Data processing
3.2.9Gene annotation
3.3Results
3.3.1 Descriptive statistics and agronomic traits of Bing4114 and L6238
3.3.2 Genetic analysis of flag leaf and panicle length and other related traits in segregating BC1F2 population
3.3.3 Correlation analysis of other related agronomic traits in segregating BC1F2 population
3.3.4 Primary mapping of the QTL qLFP9 for flag leaf and panicle length in rice
3.3.5Fine mapping of the QTL qLFP9 for flag leaf and panicle length in rice
3.3.6Candidate gene expression analysis
3.4Discussion
3.5Conclusions
General Summary
References
Appendix
Acknowledgements
List of Publications
声明
扬州大学;
