声明
Abbreviations:
Chapter 1:Introduction
1.1 What Is Ulcerative Colitis?
1.2 Prevalence of ulcerative colitis
1.3 Etiology of ulcerative colitis
1.4 Disorder of the immune system
1.5 DSS model
1.6 Role of high-altitude hypoxia on immune function
Chapter 2:Materials and Methods
2.1 Experimental design
2.2 Mice
2.3 Chemicals
2.4 Induction of Ulcerative colitis (UC)
2.5 Hypoxia induction
2.6 Clinical markers
2.7 Histopathological examinations
2.8 Measurement of Myeloperoxidase Activity
2.9 Enzyme-Linked Immunosorbent Assay (ELISA)
2.10 Isolation of lymphocytes and purification of CD4+T cells
2.11 Flow cytometry assay
2.12 Gene expression assay
2.13 Statistical analysis
Chapter 3:Results
3.1 Clinical Features
3.2 INFLAMMATORY MARKERS
3.3 Effect of high-altitude hypoxia on CD4+subtypes cells.
3.4 Effect of high-altitude hypoxia on mRNA expression.
3.5 Histological examination
Chapter 4:Discussion
Conclusion and future work
参考文献
致谢
SELF-INTRODUCTION
青海大学;
