声明
1. Introduction and Review of Literature
1.1 Research Background
1.2 Review of Literature
1.2.1 Characteristics of lead-zinc mine tailings
1.2.2 An overview of worldwide pollution by heavy metals
1.2.3 Scenario of soil pollution by heavy metals in China
1.2.4 Deleterious effects of heavy metals on human health and plants
1.2.5 Remediation technologies of heavy metal contaminated sites
1.2.6 Importance of aided-phytostabilization in mine tailings
1.2.7 Use of different amendments to facilitate phytoremediation of mine sites
1.2.8 Effect of heavy metals on enzyme activity
1.3 Research Contents
1.4 Research Hypothesis and Objectives
1.5 Technical Pathway
2. Materials and Methods
2.1 Site Description
2.2 Experimental Materials Collection
2.2.1 Pb-Zn mine tailings
2.2.2 Amendments
2.2.3 Tested plant
2.3 Experimental Methods
2.3.1 Pot experiment
2.3.2 Sample Analyses
2.4 Quality Control and Statistical Analyses
3. The Influence of Amendments on pH, EC and Bioavailable Metal Concentrations in Treated Tailings
3.1 Characterization of Studied Pb-Zn Mine Tailings and Amendments
3.2 Effect of Amendments on pH and EC of Tailings
3.3 Effect of Amendments on DTPA-extractable Pb Concentrations in Treated Tailings
3.4 Effect of Amendments on DTPA-extractable Zn Concentrations in Treated Tailings
3.5 Effect of Amendments on DTPA-extractable Cd Concentrations in Treated Tailings
3.6 Effect of Amendments on DTPA-extractable Cu Concentrations in Treated Tailings
3.7 Summary
4. Influence of Amendments on Enzyme Activities in Treated Tailings
4.1Effect of Amendments on Urease Activity in Treated Tailings
4.2Effect of Amendments on Dehydrogenase Activity in Treated Tailings
4.3Effect of Amendments on β-glucosidase Activity in Treated Tailings
4.4 Effect of Amendments on Acid and Alkaline Phophatase Activity in Treated Tailings
4.5 Effect of Amendments on Catalase Activity in Treated Tailings
4.6 Summary
5. Impact of Amendments on Amorpha fruticosa Growth, Chlorophyll Content and Metal Uptake in Plant Tissues
5.1 Impact of Amendments on Dry Biomass Yield of A. fruticosa
5.2 Impact of Amendments on Shoot Height and Root Length
5.3 Impact of Amendments on Chlorophyll Content
5.3 Impact of Amendments on Metal Uptake in Plant Tissues
5.3.1 Impact of amendments on Pb uptake in plant tissues
5.3.2 Impact of amendments on Zn uptake in plant tissues
5.3.3 Impact of amendments on Cd uptake in plant tissues
5.3.4 Impact of amendments on Cu uptake in plant tissues
5.4 Phytoremediation Efficiency of A. fruticosa
5.5 Summary
6. Effect of Amendments on Physiological Functions in Amorpha fruticosa
6.1. Concentration of Superoxide Anion (O2·-) and Malondialdehyde (MDA) in A. fruticosa Leaves Grown in Amended Tailings
6.2 Effect of Amendments on Antioxidant Enzyme Activities in A. fruticosa Leaves
6.2.1 Effect of amendments on SOD activityin A. fruticosa leaves
6.2.2 Effect of amendments on POD activity in A. fruticosa leaves
6.2.3 Effect of amendments on CAT activity in A. fruticosa leaves
6.2.4 Effect of amendments on APX activity in A. fruticosa leaves
6.2.5 Effect of amendments on PAL activityin A. fruticosa leaves
6.2.6 Effect of amendments on PPO activity in A. fruticosa leaves
6.3 Impact of Amendments on Soluble Protein and Soluble Sugar Concentration in A. fruticose Leaves
6.4 Summary
7. Conclusions and Recommendations
7.1. Conclusions
7.2 Innovations of the Study
7.3 Suggestions and Recommendations
参考文献
Appendix
Nomenclature
致谢
About The Author
Published Peer Reviewed Papers (SCI)
西北农林科技大学;
