声明
Chapter 1 Introduction
1.1 Research background
1.2 Overview of research on B. subtilis
1.3 Extraction and separation of natural products
1.4 Precursor-directed biosynthesis
1.5 The objectives and significance of this research
1.6 Research steps, methods and workflow for the project
Chapter 2 Separation and screening of soil samples
2.1 Experimental materials, reagents and equipment
2.2 Experimental methods
2.3 Screening of biological activity and HPLC analysis
2.4 Experimental results
2.5 Discussion
hapter 3 Homology analysis of 16S rDNA gene sequence of strain TJU01W1
3.1 Experimental Materials, reagents, equipment
3.2 Experimental methods
3.3 Experimental results
3.4 Discussion
Chapter 4 Preliminary studies on fermentation conditions for strain TJU01W1
4.1 Experimental Materials, reagents and equipment
4.2 Experimental methods
4.3 Culture conditions
4.4 Experimental results
4.5 Discussion
Chapter 5 Extraction, isolation, purification and structural identification of secondary metabolites from strain TJU01W1
5.1 Experimental Materials, reagents and equipment
5.2 Experimental methods
5.3 Experimental results
5.4 Discussion
Chapter 6 Precursor-Directed Biosynthesis
6.1 Experimental reagents, equipment
6.2 Experimental methods
6.3 Experimental results
6.4 Discussion
Chapter 7 Antimicrobial assay
7.1 Experimental reagents and equipment
7.2 Experimental method
7.3 Experimental results
7.4 Discussion
Chapter 8 Conclusion
参考文献
Appendices
致谢
天津大学;
