基于关联分析发掘水稻(Oryza sativa L.)幼苗中胚轴伸长长度和苗高的优异等位变异

目录

声明

TABLE OF CONTENTS

ABSTRACT

摘要

LIST OF ABBREVIATIONS

CHAPTER 1 LITERATURE REVIEW

1．1 Rice as Human Food

1．2 Rice Plant

1．3 Direct Seeded Rice(DSR)

1．4 Types of Direct-Seeded Rice

1．4．1 Dry direct seeding

1．4．2 Wet direct seeding

1．4．3 Water direct seeding

1．5 Assessment Cultivation Practices for Direct-Seeded Rice(United States，Sri Lanka,and Malaysia)

1．6 Potential Benefits Associated With Direct-Seeded Rice

1．7 Direct Seeding Traits

1．8 Molecular Marker Tools to Study Complex Traits in Rice

1．8．1 Association Genetics

1．8．2 Statistical Methods for Association Mapping

1．9 Research Objective and Technical Route

CHAPTER 2 MATERIALS AND METHODS

2．1 Plant Materials

2．2 Seed Multiplication on the Field

2．3 Mesocotyl and Shoot Length (Elongation)Evaluation in Growth Chamber

2．4 Mesocotyl Elongation Length and Shoot Length Evaluation on the Field

2．5 DNA Extraction and PCR Amplification

2．7 Genetic diversity and PIC determination

2．8 Measure of Linkage Disequilibrium

2．9 Population Structure

2．10 Identifying Molecular Markers Associated with Traits

2．11 Identifying Elite Alleles Associated with Traits

2．12 Design of Novel Parental Combinations

CHAPTER 3 RESULTS AND ANALYSIS

3．1 Descriptive Statistics and Heritability of Mesocotyl Elongation Length and Shoot Length within the Population

3．1．1 Phenotypic analysis of mesocotyl elongation length observed in the growth chamber

3．1．2 Phenotypic analysis of mesocotyl elongation observed on the soil condition(SC)，and the relationship with growth chamber condition(GCC)

3．2 Genetic Diversity of SSR Markers

3．3 Population Structure

3．4 Linkage Disequilibrium

3．5 Significant Marker-Trait Association Loci Detected and Mining of Elite Alleles for Mesocotyl Elongation Length

3．6 Allelic Variation For Mesocotyl Elongation Length Accumulated in the Topmost 10 Varieties and Lowest 10 Varieties

3．7Allelic Variation For Shoot Length Accumulated in the Topmost 10 Varieties and Lowest 10 Varieties

3．7 Phenotypic Analysis of Shoot Length

3．8 Significant Marker-Trait Association Loci Detected and Mining of Elite Alleles for SL

CHAPTER 4 DISCUSSION AND CONCLUSIONS

4．1 Discussion

4．2 Conclusions

CHAPTER 5 MAJOR INNOVATION POINTS

REFERENCES

Appendix

PUBLICATIONS

ACKNOWLEDGEMENT