论文说明
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ACKNOWLEDGMENTS
摘要
1. Introduction
2. Review of Literature
2.1 Genetic transformation of cotton
2.1.1 Genetic engineering
2.1.2 Cotton Tissue Culture
2.1.3 Agrobacterium -Mediated Cotton Transformation
2.1.4 Particle Bombardment Method of Cotton Transformation
2.1.5 In-planta transformation
2.1.6 Transformation efficiency of cotton
2.1.7 Limitations of in-vitro transformation methods
2.2 Performance of Bt cotton
2.2.1 Bollworm resistant transgenic cotton
2.2.2 The history of Bt cotton
2.2.3 Advantages of Bt Cotton over Bt spray in cotton
2.2.4 Bt cotton status
2.2.5 The performance of Bt cotton
2.2.6 Fiber quality
2.3 References
3 Optimization of Modified Agrobacterium Transfected Pollen via Pollen Tube Pathway Method for Genetic Transformation of Gossypium hirsutum L
Abstract
3.1 Introduction
3.2 Materials and methods
3.2.1 Plant materials
3.2.2 Vector and bacterial strain
3.2.3 Transformation protocol
3.2.4 Pollen germinating medium
3.2.5 Screening of cotton seedlings for bastaR tolerance
3.2.6 Polymerase chain reaction (PCR) analyses
3.2.7 Southern blot hybridization analysis
3.2.8 Agrobacterium tumefaciens contamination test
3.2.9 Progeny tests
3.3 Results and Analysis
3.3.1 Sucrose concentration and duration of incubation
3.3.2 Basta concentration
3.3.3 Herbicide Bioassay test
3.3.4 PCR analyses
3.3.5 Southern blot hybridization
3.3.6 Analyses for persistence of A. tumefaciens
3.3.7 Transformation Efficiency
3.3.8 Analysis ofT1 plants
3.4 Discussion
3.5 References
4 Experiment on Comparison of Transformation Methods for Genetic Transformation in Upland Cotton (Gossypium hirsutum L.)
Abstract
4.1 Introduction
4.2 Materials and methods
4.2.1 Agrobacterium strain and plant species
4.2.2 Transformation methods
4.2.3 Selection and screening of putative transgenic seedlings
4.2.4 Polymerase chain reaction (PCR) analyses
4.2.5 Southern blot hybridization analysis
4.2.6 Agrobacterium tumefaciens contamination test
4.2.7 Progeny tests
4.3 Results and analysis
4.3.1 Bioassay test for herbicide
4.3.2 PCR analyses
4.3.3 Southern blot hybridization
4.3.4 Analyses for persistence of A. tumefaciens
4.3.5 Efficiency of the systems
4.3.6 Analysis of T1 plants
4.4 Discussion
4.5 References
5 Insect resistant transgenic cotton: Effects of foreign genes on plant growth, lint yieldand fiber quality oftransgenic cotton
Abstract
5.1 Introduction
5.2 Materials and methods
5.2.1 Experiment location
5.2.2 Plant cultivars
5.2.3 Design of experiment
5.2.4 Plant protection
5.2.5 Field observations for growth and yield components
5.2.6 Fiber quality
5.2.7 Statistical analysis
5.3 Results and analysis
5.3.1 Yield and yield components
5.3.2 Growth components
5.3.3 Bollworm larvae infestation
5.3.4 Fiber quality
5.4 Discussion
5.4.1 Yield advantage
5.4.2 Fiber quality
5.5 References
6 Insect Resistant Transgenic Cotton: Effects of Monocrotophos Insecticide Sprayingon Growth, Yield, Fiber Quality and Plant Resistance to Verticillium Wilt
Abstract
6.1 Introduction
6.2 Materials and method
6.2.1 Materials
6.2.2 Methods
6.3 Results and Analysis
6.3.1Theeffectsofthemonocrotophossprayingonthegrowthand development of the transgenic cotton plants
6.3.2 The effects of the monocrotophos spraying on the yield and fiberquality of the transgenic cotton plants
6.3.3. The effects of the monocrotophos spraying on the Verticillium wiltresistance of the transgenic cotton plant:
6.4. Discussion:
6.5 References
