Modulation of Microglia Function by Dopamine and Serotonin

目录

声明

Chapter 1 Introduction

1.1 Microglia

1.1.1 The origin

1.1.2 The microglial morphology and activation

1.1.3 The microglial function

1.1.4 Microglia and disease

1.2 Cell signaling pathway and cell chemotaxis

1.2.1MAPK cell signaling.

1.2.2 Paxillin and cofilin

1.3 Hypothesis and Aims

Chapter 2Experimental Materials and Methods

2.1Experiment materials

2.1.1 Cell

2.1.2 Antibody and reagent

2.1.3 Instrument

2.2 Solution preparation

2.3. Method

2.3.1 Cell culture

2.3.2 Cells subculture:

2.3.3 Cryopreservation of cells

2.3.4 Cells recovery:

2.3.5Western blotfor cellular protein levelsdetection:

2.3.6 Transformation

2.3.7BCA for total protein level detection

Chapter 3 Result

3.1ERK1/2 activation was not significantly altered upon DA or 5-HT stimulation in Bv2 microglia cells.

3.2DA and 5-HT induce the activation of p38 MAPK in a time-dependent manner in resting microglia.

3.3Activation of p38 MAPK by DA cannot be blocked with DA receptor antagonists.

3.4Activation of p38 MAPK by DA or 5-HT can be blocked with decynium.

3.5Activation of p38 MAPK results in the phosphorylation of paxillin Ser83

3.6Phagocytosis and F-actin stress fibers are downregulated in DA-treated microglia

Chapter 4 Discussion

参考文献

致谢