声明
Chapter 1. Introduction
1.1 Nanocarriers as drug delivery systems
1.2Introduction to stimuli-responsive nanocarriers
1.2.1Redox-responsive nanocarriers
1.2.2Other types of stimuli-responsive nanocarriers
1.3Approaches formonitoring drug release from nanocarriers
1.3.1Traditional methods for monitoring drug release
1.3.2Recent advances in monitoring drug release
1.4Introduction to FRET technique
1.4.1FRET principle
1.4.2FRET materials
1.4.3Pharmaceutical application of FRET
1.5Recent advances of AIE molecules
1.5.1AIE mechanisms
1.5.2AIEgens
1.5.3AIE applications
1.6Hypothesis and aim of the project
Chapter 2. Synthesis of amphiphilic polymer-drug conjugated
2.1Materials and instruments
2.1.1Materials
2.1.2Instruments
2.2Synthesis and characterization of TPE-OH
2.2.1Synthesis of TPE-OH
2.2.2Results and discussion
2.3Synthesis and characterization of TPE-COOH
2.3.1Synthesis of TPE-COOH
2.3.2Results and discussion
2.4Synthesis and characterization of Cur-SS
2.4.1Purification of curcumin
2.4.2Synthesis of Cur-SS
2.4.3Results and discussion
2.5Synthesis and characterization of Lys(Z)-NCA
2.5.1Synthesis of Lys(Z)-NCA
2.5.2Results and discussion
2.6Synthesis and characterization of mPEG-PLys(Z)
2.6.1Synthesis of mPEG-PLys(Z)
2.6.2Results and discussion
2.7Synthesis and characterization of mPEG-PLys(Z)-TPE
2.7.1Synthesis of mPEG-PLys(Z)-TPE
2.7.2Results and discussion
2.8Synthesis and characterization of mPEG-PLys-TPE
2.8.1Synthesis of mPEG-PLys-TPE
2.8.2Results and discussion
2.9Synthesis and characterization of mPEG-PLys(Cur)-TPE
2.9.1Synthesis of mPEG-PLys(Cur)-TPE
2.9.2Results and discussion
Chapter 3. Preparation and characterization of polymeric micelles
3.1Materials and instruments
3.1.1Materials
3.1.2 Instruments
3.2Preparation of polymeric micelles
3.3Characterization of polymeric micelles
3.3.1 UV-vis spectra of polymeric micelles
3.3.2Particle size analysis of polymeric micelles
3.3.3Stability of polymeric micelles
3.3.4Fluorescence spectra of polymeric micelles
3.3.5 AIE performance of polymeric micelles
Chapter 4. Drug release from the redox-responsive micelles
4.1 Materials and instruments
4.1.1Materials
4.1.2Instruments
4.2Preparation of sample stocks
4.3Performance of micelles in response to GSH trigger
4.3.1Kinetic emission spectra of TPE and Cur
4.3.2Kinetic absorption spectra
4.3.3Hydrodynamic size analysis
4.3.4Results and discussion
4.4Micelles disassembly in response to TCEP trigger
4.4.1Kinetic emission spectra of TPE and Cur
4.4.2 Kinetic absorption spectra
4.4.3Hydrodynamic size analysis
4.4.4Results and discussion
Chapter 5. Intracellular evaluation of redox-responsive micelles
5.1Materials and instruments
5.1.1Materials
5.1.2Instruments
5.2Cells viability assays
5.2.1The principle of cell viability assay
5.2.2The experimental process
5.2.3Results and discussion
5.3Cellular uptake
5.3.1The experimental process
5.3.2 Results and discussion
5.4Cumulative drug release in cells
5.4.1The experimental process
5.4.2Results and discussion
5.5Quantitative flow cytometry analysis
5.5.1The experimental process
5.5.2Results and discussion
Chapter 6. Conclusions and prospects
6.1Conclusions
6.2 Prospects
参考文献
Publication
致谢