论文说明：List of Figures and List of Tables and Abbreviations
声明
Abstract
摘要
Preface
Chapter Ⅰ Analysis of Microsatellite Markers Derived from Expressed Sequence Tags (EST) of Sweet Potato
1. Introduction
2. Materials and Methods
2.1 Genetic material
2.2 EST-derived primers and reaction condition
2.3 Estimation of polymorphic information content (PIC) and diversity
3. Results
3.1 Transferability and polymorphism
3.2 Cluster analysis involving 52 genotypes
4. Discussion
4.1 Polyploidy of sweet potato
4.2 Transferability and polymorphism of sweet potato EST-SSR
4.3 Application of EST-SSR in the germplasm management
4.4 The limitations of EST-SSR of sweet potato
5. Conclusion
6. References
Chapter Ⅱ Assessment of the Diversity of Sweet Potato by EST-SSR Markers
1. Introduction
2. Materials and Methods
2.1 Genetic materials
2.2 EST-derived primers and reaction conditions
2.3 Gel scoring and data analysis
3. Results
3.1 Regional distribution of allelic diversity
3.2 Cluster analysis
4. Discussion
4.1 Hypothesized origin center of sweet potato
4.2 Prehistoric dispersal of sweet potato
5. Conclusion
6. References
Chapter Ⅲ Establishment of a Rapid, Simple and Efficient Agrobacterium-mediated Transformation Method of Sweet Potato
1. Introduction
2. Materials and Methods
2.1 Plant materials
2.2 Bacterial cultures and plasmids
2.3 Genetic transformation procedure
2.4 Verification of resistance to kanamycin
2.5 Plant DNA isolation
2.6 PCR analysis
2.7 Southern hybridization
2.8 RT- PCR analysis
3. Results
3.1 Organogenesis regeneration of putative transformed events
3.2 Tissue culture-based assay for true transformants
3.3 Molecular characterization of transformed sweet potato plants
3.4 Expression of the nptⅡ gene
4. Discussion
4.1 de novo organogenesis and hormones
4.2 The "escapes" and tissue culture assay
4.3 The new transformation system via de novo organogenesis
5. Conclusion
6. References
ChapterⅣ Somatic Embryogenesis of an Africa Sweet Potato (Ipomoea batatas (L.) Lam.) Variety
1. Introduction
2. Materials and Methods
2.1 Plant materials
2.2 Treatments
2.3 Experiments design
3. Results and Discussion
3.1The statistical analysis of variance
3.2 Effect of eultivar differences
3.3 Effect of auxins on embryogenesis callus formation
4. Conclusion
5. References
ChapterⅤ Agrobacterium-mediated Transformation Efficiency of Some Chinese Sweet Potato Varieties
1. Introduction
2. Materials and Methods
2.1 Plant materials
2.2 Strain
2.3 Plasmid
2.4 Genetic transformation procedure
2.5 Verification of resistance to kanamycin
2.6 Plant DNA isolation
2.7 PCR analysis
3. Results and Discussion
3.1 Transformation efficiency of different sweet potato cultivars
3.2 Kanamycin resistance evaluation of hLFc- transgenic lines
3.3 PCR analysis of transgenic lines
4. Conclusion
5. References
Literature Review: Advance in the Study of Sweet Potato
1. World distribution of sweet potato
2. Botany of sweet potato
3. Diffusing of sweet potato
4. Genebank of sweet potato maintained at CIP
4. Utilization: food, cash, feed and processed products
6. The advance research of EST-SSR markers
7. Genetic transformation and regeneration research in sweet potato
8. References
Acknowledgements
Appendix
