文摘
英文文摘
论文说明:ABREVIATIONS/TABLE OF FIGURES
声明
外文摘要
致谢
摘要
PREFACE
INTRODUCTION ON FLUORESCENCE
Ⅰ.1 General Definitions:Characteristics of fluorescence and the fluorescence microscope
Ⅰ.1.1 Photoluminescence process
Ⅰ.1.2 Steady-state characteristics of fluorescence emission
Ⅰ.1.3 Time-resolved characteristics of fluorescence emission,fluorescence lifetime
Ⅰ.2 Fluorescence anisotropy and instrumental considerations
Ⅰ.2.1 Steady-state fluorescence anisotropy:definition and principle
Ⅰ.2.2 Time-resolved fluorescence anisotropy:definition and principle
Ⅰ.2.3 Instrumentation and design of pulse fluorometers
Ⅰ.2.4 Examples of application of fluorescence anisotropy in the field of biology
Ⅰ.3 Fluorescence correlation spectroscopy
Ⅰ.3.1 Fluorescence correlation spectroscopy:definition
Ⅰ.3.2 Conceptual basis of Fluorescence correlation spectroscopy and determination of diffusion constant
Ⅰ.3.3 Fluorescence cross-correlation spectroscopy:definition and conceptual basis
Ⅰ.3.4 Instrumentations and methods of FCS and FCCS
Ⅰ.3.5 Example of application of FCS and FCCS in the field of biology
MOTIVATION AND OBJECTIVE
MATERIAL AND METHODES
Ⅱ.1 Reagents and buffers
Ⅱ.1.1 HIV integrase related buffers
Ⅱ.1.2 RecQ helicase related buffers
Ⅱ.2 Protein and oligonucleotides purification
Ⅱ.2.1 HIV integrase purification
Ⅱ.2.2 RecQ helicase purification
Ⅱ.2.3 Oligonucleotides purification and preparation
Ⅱ.3 Enzymatic activity measurements
Ⅱ.3.1 Steady-state fluorescence based activity measurements
Ⅱ.3.2 Fluorescence cross-correlation measurements
Ⅱ.3.3 Quantified maximum entropy method of time-resolved fluorescence data(MEM)
APPLICATION OF FCCS TO STUDY THE ENZYMATIC KINETICS OF RECQ HELICASE
Ⅲ.1 General introduction to the relationship between DNA and helicases
Ⅲ.1.1 Structure and function of DNA
Ⅲ.1.2 The function of helicase in cellular processes involved with DNA
Ⅲ.1.3 Helicase characteristics and helicase deficiency diseases
Ⅲ.2 E.Coli RecQ Helicase Unwinding Activity as Monitored by Dual Color Fluorescence Correlation Spectroscopy
Ⅲ.2.1 Introduction
Ⅲ.2.2 Manuscript
Ⅲ.2.3 Conclusion
Ⅲ.3 Human RecQ5β Unwinding and Strand-Annealing Activity as Monitored by Dual Color Fluorescence Correlation Spectroscopy
Ⅲ.3.1 INTRODUCTION
Ⅲ.3.2 MATERIALS AND METHODS
Ⅲ.3.3 RESULTS
Ⅲ.3.4 DISCUSSION
Ⅲ.3.5 PROSPECTIVE
Ⅲ.3.6 FIGURES AND LEGENDS
APPLICATION OF FLUORESCENCE ANISOTROPY TO STUDY ENZYMATIC KINETICS OF HIV-IN
Ⅳ.1 The basic biology of retroviruses
Ⅳ.1.1 Structural taxonomic for the family of retroviruses
Ⅳ.1.2 Replication cycle and pathology of HIV-virus
Ⅳ.1.3 Introduction to anti-retroviral treatments
Ⅳ.1.4 Structure and function of HIV-integrase
Ⅳ.2 The G140S mutation in HIV integrases from raltegravir-resistant patients rescues catalytic defect due to the resistance Q148H mutation
Ⅳ.2.1 Introduction
Ⅳ.2.2 Manuscript
Ⅳ.2.3 Conclusion
APPLICATION OF PHOTOLUMINESCENCE DECAY TO CHRACTERIZE QDS FLUORESCENCE PROPERTIES
Ⅴ.1 General introduction to the semiconductor nanoparticles
Ⅴ.1.1 Definition of quantum dots
Ⅴ.1.2 Spectral properties of quantum dots
Ⅴ.1.3 Solubilization and biological applications of quantum dots
Ⅴ.2 Multi-exponential Photoluminescence Decay of CdTe Quantum Dots upon One and Two Photon Excitation
Ⅴ.2.1 INTRODUCTION
Ⅴ.2.2 MATERIALS AND METHODS
Ⅴ.2.3 RESULTS
Ⅴ.2.4 DISCUSSION AND CONCLUSION
Ⅴ.2.5 PROSPECTIVE
Ⅴ.2.6 FIGURES AND LEGENDS
GENERAL CONCLUSION
REFERENCES