文摘
英文文摘
声明
外文摘要
Acknowledgement
Outlines
Chapter 1 Introduction
1.1 Cell Patterning
1.1.1 Cells and stem cells
1.1.2 Cell-material interaction
1.1.3 Previous works
1.2 Microfluidics and Lab-on-a chip system
1.2.1 History recall of Microfludics
1.2.2 Fundamental notions of Microfluidics
1.2.3 On chip cell culture and differentiation
1.2.4 Previous works
1.3 Objective of this work
References：
Chapter 2 Cell patterning
2.1 Microfabrication techniques
2.1.1 Photolithography
2.1.2 Soft lithography
2.1.3 Microcontact printing(μCP)
2.1.4 Nanoimprint lithography(NIL)
2.2 PDMS degas induced cell patterning
2.2.1 PDMS mold fabrication
2.2.2 PDMS degas induced patterning
2.2.3 Cell patterning
2.2.4 Results and discussions
2.2.5 Conclusions
2.3 High resolution cell patterning
2.3.1 General lithography method
2.3.2 Culture patterning
2.3.3 Results and discussions
2.3.4 Conclusions
2.4 Single cell pattern in microfluidic chip
2.4.1 The fabrication of the patterned chip
2.4.2 The Poly-Lysine-FITC pattern
2.4.3 Single cell pattern in chip
2.5 Conclusions
References:
Chapter 3 On-chip cell culture：case of ES cells
3.1 Introduction
3.2 Microfluidic device fabrication for mES cells
3.3 Simple devices for MEFs.
3.4 On-chip ES culture and differentiation
3.4.1 mES Cell culture
3.4.2 EB formation and differentiation
3.4.3 Seeding MEF cells on micro-channel-well system
3.5 Immunocytochemistry
3.6 Seeding MEFs on the different ratios of PDMS substrates
3.7 Results and discussions for ES cells
3.7.1 Cell adhesion depends on different substrates
3.7.2 ES co-culture with MEF onto micro-channel-well system
3.7.3 ES-derived neural differentiation on micro-channel-well system
3.8 Results and discussions for MEFs on PDMS substrates
3.9 Conclusion
References：
Chapter 4 On-chip cell culture：case of MSCs
4.1 Introduction
4.2 Microfluidic device fabrication
4.3 On-chip cell culture
4.4 RMSCs culture in the three microfluidic devices
4.4.1 Characterization of microfluidic devices for RMSCs
4.4.2 RMSCs in microfluidic device Ⅰ
4.4.3 RMSCs in microfluidic device Ⅱ
4.4.4 RMSCs in microfluidic device Ⅲ
4.4.5 RMSCs in culture flask
4.4.6 Comparison of the three devices
References
Chapter 5 On-chip cell differentiation：case of MSCs
5.1 Introduction
5.2 Microfluidic device fabrication
5.3 Differentiation protocol
5.3.1 Adipogenic differentiation
5.3.2 Neural differentiation
5.3.3 Characterization techniques
5.4 Results and discussions
5.4.1 Adipogenic differentiation
5.4.2 Neural differentiation
5.5 Conclusion
References：
Chapter 6 Towards a cellular network formation
6.1 Introduction
6.2 Experiments
6.2.1 Microcontact printing pattern for RMSCs
6.2.2 The microwell-network for mES cells.
6.3 Microcontact printing PLL Pattern for RMSCs
6.3.1 Microcontact printing PLL Pattern
6.3.2 RMSCs network pattern
6.3.3 Other patterns
6.3.4 Concluding remarks
6.4 Mirowell-network for mES cells
6.4.1 the microwell-network
6.4.2 mES-derived neurons in the microwell network.
6.5 First try on electrode chip for the signal transduction
References
Summary and Perspective
Publication list of this thesis work
APPENDIX
