水稻86,136EST的功能注释、表达图谱比较及可变剪接形式分析

摘要

运用在基因组项目中成熟起来的高通量测序技术使得EST测序项目及其数据分析已经成为基因识别的有力工具.EST数据分析可以为我们提供关于基因功能与表达的重要信息,特别是在与基因组序列一同分析时,我们可以得到更有价值的信息.水稻是世界上最重要的粮食作物之一.我们对86,136条水稻EST序列进行测序,并且对其基因功能、表达谱和水稻的剪接形式变化进行了分析.

目录

摘要

1 Introduction

2 Text

2.1 Materials and Methods

2.1.1 Library information

2.1.2 Sequence quality check and sequencing progress monitor

2.1.3Clustering and contig checkv

2.1.4 Complete ORF finding

2.1.5 Function assignment and classification

2.1.6 Expression profile analysis

2.1.7 Altemative splicing database and prediction

2.1.8 General-purpose EST analysis pipeline

2.2 Results

2.2.1 Library information and quality check

2.2.2 Sequence quality check and sequencing progress monitor

2.2.3 Clustering and contig check

2.2.4 Function assignment and classification

2.2.5 Expression profile analysis

2.2.6 Alternative splicing database and prediction

2.3 Discussion

2.3.1 Summary of our EST analysis results

2.3.2 Extension of the EST pipeline to genome pipeline

2.3.3 Automatic annotation?

3 Reference

4 Appendence

4.1 Acknowledgement

4.2 Other works

4.3 Publications

附件

声明