文摘
英文文摘
Acknowledgment
Chapter 1: Introduction
1.1 Brief introduction
1.2 Life history
1.3 Introduction to aquaculture
1.3.1 Techniques and equipment
1.3.2 Broodstock
1.3.3 Spawning and larval rearing
1.3.4 Grow-out
1.3.5 Feeding
1.3.6 Grow-out facilities
1.3.7 Harvesting
1.4 Recent development in abalone farming in SA
Chapter 2: Background
2.1 Introduction to cryopreservation and its uses
2.2 Advantages of cryopreservation in shellfish
2.3 Cryopreservation in abalone
2.3.1 Research in China
2.3.2 Research in New Zealand
2.3.3 Research in Australia
Chapter 3: Material and methods
3.1 Chemicals and equipments
3.2 Selection of spawners and collection of gametes
3.3 Optimization of parameters
3.3.1 Selection of the type and concentration of cryoprotectant
3.3.2 Selection of optimal pre-freezing temperature
3.3.3 Effect of sperm volume on cryopreservation
3.3.4 Selection of sperm concentration
3.3.5 Cold storage of greenlip abalone sperm
3.3.6 Thawing temperature
3.3.7 Selection of egg/milt ratio
3.4 Experimental procedures
Chapter 4: Results
4.1 Determination of type and concentration of cryoprotectant
4.2 Selection of optimal pre-frezing temperature
4.3 Effect of sperm volume on cryopreservation
4.4 Effect of sperm concentration
4.5 Cold storage of greenlip abalone sperm
4.6 Thawing temperature
4.7 Comparison of milt/egg ratio
Chapter 5: Discussion
5.1 Concentration of cryoprotectant
5.2 Selection of pre-freezing temperature
5.3 Effect of refrigeration of sperm prior to immersion in LN
5.4 Thawing temperature
5.5 Comparison of milt/egg ratio
5.6 Evaluation of larval development
Chapter 6: Conclusion
References
Appendix
