Abstract
摘要
Table of Contents
List of Abbreviations
1 Introduction
1．1 Transgenic Rice
1．1．1 Rice Transformation
1．2 The history of Heterotrimeric G-Proteins
1．2．1 What are Heterotrimeric G-protein subunits?
1．2．2．Heterotrimeric G-protein subunits review
1．3 CRISPR／Cas9 system
1．3．1．CRISPR／Cas9 System
1．3．2．Advantage of using CRISPR／Cas9 system
2 Materials and Methods
2．1．Materials
2．1．1 Plant material
2．1．2 Strains and vectors
2．1．3 Experimental reagent
2．1．4 Medium and hormone formulation
2．1．5 Common molecular biology laboratory equipments
2．2 Methods
2．2．1 Bioinformatics analysis
2．2．2 Vector construction
2．2．3 Agrobacterium Transformation procedure
2．2．4 Tissue culture
2．2．5 DNA isolation and PCR to confirm rggl and rgg2 mutants
2．3 Gene function and analysis of the phenotype
2．3．1 Plant material，seed germination assay and potassium treatment
2．3．2 Gene expression analysis
3 Resuits
3．1 Rice Tralasformation
3．1．1 Confirmation of RGG2 overexpressed
3．1．2 Confirmation of rgg1 and rgg2 mutants
3．1．3 The sequence test of transgenic plants for rgg1 and r992 mutants
3．2．Phenotype analysis of rgg1 mutants and RGG2(OE)lines
3．3 Potassium treatment analysis
3．3．1 Phenotype of K+treatment
3．3．2 Expression level of genes related to potassium
3．4 Different level of trace elements
4 Discussion
References
6 Appendix
Acknowledgement
声明
扬州大学;
