声明
摘要
ABSTRACT
DEDICATIoN
CONTENTS
List of Figures
List of Tables
CHAPTER 1 Literature Review
1．1．Taxonomy and a General Introduction to Mycoplasmas
1．2．Ecology and Pathogenesis of Mycoplasma
1．2．1 Evasion of the Immune system
1．2．2 Interaction with the Host Cells and Cytopathology
1．2．3 M.hyorhinis Major Immunogen，P37
1．3．Etiology
1．4．Epidemiology
1．5．Clinical Presentation of M. hyorhinis infection in swine
1．6．Diagnostic techniques for the Detection of M．hyorhinis
1．6．1 Diagnosis
1．6．2 Macroscopic and microscopic examination
1．6．3 Fluorescent antibody and immunohistochemistry assays
1．6．4 Culture
1．6．5 Serology
1．6．6 Polymerase chain reaction (PCR)
1．7．Biosecurity and an integrated approach for the Management of M. hyorhinis
1．8．The Swine Industrv in Kenya and China
1．9．Problem Statement
1．10 Rationale
1．11 Hypothesis
1．12 Objectives of the study
CHAPTER 2 Detection of Respiratory Pathogens M. hyorhinis and M. hyopneumoniae by Nested PCR in porcine Nasal Swabs From clinically Infected Herds
2．1 Introduction
2．2 Materia ls a nd Methods
2．2．1 Animals
2．2．2 Sample preparation
2．2．3 Nested PCR
2．2．4 Statistical analvsls
2．3 Results
2．4 Discussion
CHAPTER 3 Detection，Isolation and Identification of M. hyorhinis and Assessing the Antimicrobial Profiles of the Isolates
3．1 Detection，Isolation and Identification of M. hyorhinis
3．1．1 Introduction
3．1．2 Materials and Methods
3．1．3 Results
3．2 Isolation of M. hyorhinis Strains
3．2．1 Introduction
3．2．2 Materials and Methods
3．2．3 Results
3．2．4 Discussion
3．3．The In Vitro assessment of the AntimicrobiaI Profiles of 8M. hyorhinis Isolates Against 12 Antimicrobial Agents
3．3．1 Introduction
3．3．2 Materials and Methods
3．3．3 Results
3．3．4 Discussion
CHAPTER 4 Immunogenicity Testing in Mice and Determination of Antibody Titers by IonIndirect-ELISA
4．1 Introduction
4．1．1 Freund’s adjuvant
4．1．2 Immunoglobulins
4．2 Materials and Methods
4．2．1 Antigen Preparation
4．2．2 Quantification of Antigen by CCU
4．2．3 Quantification of Antigen by BCA
4．2．4 Quantification of Antigen using qPCR
4．2．5 Results
4．2．6 Immunization
4．2．7 Detection of serum IgG antibody
4．2．8 Statistical Analysis
4．3 Results
4．4 Discussion
Chapter 5 Primary Study of Infection and Development of a Challenge Model for M. hyorhinis-Induced Pathogenesisin Swine
5．1 Introduction
5．2 Materials and Methods
5．2．1 Animals
5．2．2 Experiment 1
5．2．3 Experiment 2
5．2．4 Experiment 3
5．2．5 Sample collection and Detection
5．2．6 Histopathological examination
5．3 Results
5．3．1 Experiment 1
5．3．2 Experiment 2
5．3．3 Experiment 3
5．4 Discussion
Chapter 6 Virulence Screening of M. hyorhinis Strains through Mortality Index of experimentally infected Chicken Embryos
6．1 Introduction
6．2 Materials and Methods
6．2．1 Quantification of the strainsby CCU
6．2．2 Inoculation
6．2．3 Detection by Culture，PCR and Histopathology
6．2．4 Statistical analysis
6．3 Results
6．3．1 Mortality
6．3．2 Clinical Presentation
6．3．3 Detection by Culture and PCR
6．3．4 Histopathology
6．4 Discussion
References
Overall Conclusion
Innovation
List of Publications
Acknowledgements
ABBREVIATIONS
Appendix
