声明
Table of Contents
ABSTRACT
摘要
ABBREVIATIONS
CHAPTER 1 Literature Review and Study Plan
1 General review of Blunt snout bream in aquaculture
2 Fish nutrition，feeds and health
3 The function of dietary nutrients in fish nutrition
4 Amino acids
4．1 Utilization of amino acids
4．2 The function of amino acids
4．3 Essential amino acids requirement of fish(EAAs)
4．4 Histidine
5 Nutrition studies about blunt snout bream
6 Hypothesis and aims of the study
7 Contents of the study
CHAPTER 2 Dietary histidine requirement of juvenile blunt snout bream(Megalobrama amblycephala)
1 Introduction
2 Materials and methods
2．1 Diets preparation
2．2 Fish husbandry
2．3 Sample collection
2．4 Calculations
2．5 Proximate chemical composition analys
2．6 Plasma biochemical assay
2．7 Real-time PCR
2．8 Statistical analysis
3 Results
3．1 Growth performance and feed utilization
3．2 Whole body composition
3．3 Plasma biochemical composition
3．4 Relative expression of IGF-1 in the liver
4 Discussion
4．1 Effect of histidine on growth performance and body composition
4．2 Effect of histidine on proximate chemical composition
4．3 Effect of histidine on the relative gene expression of IGF-1
5 Conclusion
CHAPTER 3 Effects of dietary histidine level on the blood hematologyof juvenile blunt snout bream(M.amblycephala)
1 Introduction
2 Materials and methods
2．1 Diets preparation
2．2 Fish husbandry
2．3 Sample collection
2．4 Blood Hematology measurement
2．5 Plasma parameters measurement
2．6 Statistical analysis
3 Results
3．1 Effect of dietary histidine level on the hematological parameters
3．2 Effect of dietary histidine level on the plasma enzyme activity
4 Discussion
4．1 Effect of dietary histidine level on the blood hematology
4．2 Effect of dietary histidine level on the biochemical responses
5 Conclusion
CHAPTER 4 Effects of dietary histidine level on gene expression of juvenile snout bream(M.amblycephala)
1 Introduction
Conclusion of the whole study
References
Appendix
Acknowledgements
Meeting Attended and Publications
