声明
摘要
Abstract
符号说明
CHAPTER 1 Review of Related Literature
1．1 Background
1．2 Biodiesel from high oil content microalgae---the best one for these issues
1．3 Feedstock of biodiesel
1．3．1 First generation biodiesel feedstock
1．3．2 Second generation biodiesel feedstock
1．3．3 Third generation biodiesel feedstock
1．4 Microalgae for Carbon dioxide capture
1．4．1 Global warming
1．4．2 Physical methods
1．4．3 Chemical methods
1．4．4 Biological methods
1．5 Advances of microalga-based systems for carbon dioxide fixation and biofuels-producing
1．5．1 Up-stream manipulations
1．5．2 Middle-stream manipulations
1．5．3 Down-stream manipulation
1．6 Bottlenecks exist at present that preclude the full industrial exploitation of microalgal cells
1．7 The purpose and contents of the article
CHAPTER 2 How does simulated flue gases affect on the growth of Chlorella
2．1 Introduction
2．2 Materials and methods
2．2．1 Reagents and Equipments
2．2．2 Strain and culture conditions
2．2．3 Growth analysis
2．2．4 Effect of pH on the growth of Chlorella sorokiniana CS-01
2．2．5 Investigation on dry weight
2．2．6 Paraffin section and observation of microalgal morphology
2．3 Results and discussion
2．3．1 Effect of carbon dioxide on growth of Chlorella
2．3．2 Effect of carbon dioxide on the morphology of Chlorella sorokiniana CS-01
2．3．3 Effect of pH on cell growth
2．3．4 OD-t curve determination by UV-spectrophotometer
2．4 Conclusions
CHAPTER 3 How does simulated flue gases affect on the lipids accumulation and lipids composition of Chlorella sorokiniana CS-01
3．1 Introduction
3．2 Materials and methods
3．2．1 Reagents and Equipments
3．2．2 Lipid extraction
3．2．3 the analysis of lipids compositon
3．3 Results and discussion
3．3．1 Comparison of the four methods for extracting microalgal lipid
3．3．2 The effect of carbon dioxide concentration on lipids accumulation of Chlorella CS-01
3．3．3 The effect of carbon dioxide concentration on fatty acid composition of Chlorella CS-01
3．4 Conclusions
CHAPTER 4 Effect of simulated flue gases on the expression of three genes related to CO2 fixation and fatty acid synthesis
4．1 Introduction
4．2 Materials and methods
4．2．1 Major reagents and equipments
4．2．2 Real-Time PCR assays
4．3 Results and discussion
4．3．1 Total RNA extraction and analysis
4．3．2 Extraction of genome DNA and 18SrRNA amplification for building standard curve
4．3．3 Real time PCR
4．3．4 Differential expression of genes detected by RT-PCR
4．4 Conclusions
CHAPTER 5 General Conclusions and Research Prospects
References
Acknowledgement
Research achievements during three years’ postgraduate career
APPENDIX Statistics analysis of all the dates obtained from the experiments in this study
