文摘
英文文摘
声明
Chapter 1 Introduction
1.1 Whole Plant Level
1.1.1 Chemical signaling
1.2 Molecular Level
1.2.1 Perception of drought stress
1.2.2 ABA dependent signaling in drought stress
1.2.3 ABA-independent signaling in drought stress
1.3 Metabolic network in drought stress response
1.4 Research background and purpose
Chapter 2 Soil water threshold range of chemical signals and drought tolerance was mediated by ROS homeostasis in winter wheat during progressive soil drying
2.1 Introduction
2.2 Materials and Methods
2.2.1 Plant materials and growth conditions
2.2.2 Water treatments
2.2.3 Harvest
2.2.4 Gas exchange and relative leaf water content measurement
2.2.5 Comparison of drought tolerance among cultivars
2.2.6 ROS concentration
2.2.7 Enzyme assays
2.2.8 AsA and GSH content
2.2.9 Statistical analysis
2.3 Results
2.3.1 Soil water relations, stomatal conductance and leaf relative water content
2.3.2 ROS, enzyme and non-enzyme antioxidants during progressive soil water drying
2.3.3 Drought tolerance
2.4 Discussion
Chapter 3 A novel pathway regulate ABA biosynthesis and ABA signaling genes under osmotic stress condition
3.1 Introduction
3.2 Material and Methods
3.2.1 Isolation of red1 Mutant
3.2.2 Plant Material and Growth Conditions
3.2.3 Physiological Assay
3.2.4 Genetic Mapping and Complementation
3.2.5 RNA Analysis
3.2.6 Microanay Analysis
3.3 Result
3.3.1 Isolation of Arabidopsis mutants with altered ABA accumulation and reduced NCED3 expression
3.3.2 Reduced stress-responsive gene regulation by Cold, ABA, osmotic stress and salt in redl mutant
3.3.3 The red1 Mutation Enhances ABA and Osmotic Stress Sensitivity in Seeds and Vegetative Tissues
3.3.4 redl plants show higher transpiration rates and reduced drought resistance
3.3.5 Map-based cloning of the RED1/BDG gene
3.3.6 BDG1 expression is induced by osmotic stress and ABA treatments
3.3.7 BDG1 positive regulates the expression of ABA biosynthesis genes under osmotic stress treatment
3.3.8 BDG1 regulate the expression of ABA signaling genes
3.3.9 Cuticle formation regulate ABA biosynthesis genes and ABA signaling genes under osmotic stress treatment
3.4 Discussion
References
致谢
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