蛋白质氨基酸的拉曼光谱、表面增强拉曼散射（SERS）研究

摘要

本文对蛋白质氨基酸的拉曼光谱、表面增强拉曼散射（SERS）进行了研究。文章将拉曼光谱及SERS应用于研究20种蛋白质氨基酸中的几类极有代表性的氨基酸：蛋氨酸、亮氨酸、异亮氨酸、苯丙氨酸和色氨酸。分别获得其拉曼光谱及在两种基底表面的SERS。根据SERS选律和机理，推测了它们在不同基底表面的吸附状态及其不同浓度pH值下的变化规律。另外，结合拉曼和红外光谱探讨了亮氨酸和异亮氨酸与九芴甲氧羰基(Fmoc)的复合物分子内对应基团的振动情况。

目录

文摘

英文文摘

独创性声明及学位论文版权使用授权书

Segment Ⅰ Introduction

1. Introduction of Raman spectroscopy and SERS

2. The study of the amino acids by Raman spectroscopy and SERS

SegmentⅡSegmentⅡThe Study of Proteinie Aminno Acids by FT-Raman Spectroscopy and SERS

Chapter1. FT-Raman spectra of amino acids and SERS adsorbed on silver nanopartiele

1. FT-Raman spectrum and SERS of methionine

2. FT-Raman spectra and SERS of leucine and isoleucine

3. FT-Raman spectra and SERS of aromatic amino acids

Chapter 2. SERS of Leu and Isle absorbed on surface of gold/silver core-shell nanopartiele

1. Introduction

2. Experimental

3. Results and discussion

4. Conclusions

5. References

附图

Chapter 3. FTIR FT-Raman study of Fmoe-leu.Fmoc-Isle

1. Introduction

2. Experimental

3. Results and discussion

4. Conclusions

5. References

Conclusion

Appendix

Acknowledgments