声明
摘要
Abstract
TabIe of Contents
List of Tables
List of Figures
CHAPTER 1.INTRODUCTION
1.The honeybee
1.1 History of Beekeeping Industry
1.2 Beekeeping Industry in China
1.3 Economic and ecologic importance of honeybees
1.4 Honeybee colony organization and caste dependent roles
1.5 Anatomy of the Honeybee
CHAPTER 2.QUALITATIVE AND QUANTITATIVE ANTENNAL PROTEOME COMPARISON OF ITALIAN HONEYBEES(APIS MELLIFERA)ACROSS DIFFERENT AGES
2.1 Summary
2.2 Introduction
2.3 Objective
2.4 Materials and Methods
2.4.3 Protein extraction and digestion
2.4.5 LC-MS/MS analysis
2.4.6 Protein identification and quantification of abundance
2.4.7 Bioinformatics analysis
2.5 Results
2.5.1 Qualitative antennal proteome comparison of adult ITB workers across three time points
2.5.2 Quantitative antennal proteome comparison of adult worker ITBs across three time points
2.6 Discussion
2.6.1 Olfactory function of adult worker bees requires shared antennal proteome
2.6.2 Antennal proteome dynamics coordinate age-related behavioural physiology of adult worker bees
2.7 Conclusion
CHAPTER 3.QUALITATIVE AND QUANTITATIVE ANTENNAL PROTEOME COMPARISON OF ROYAL JELLY BEES (APIS MELLIFERA LIGUSTICA)ACROSS DIFFERENT AGES
3.1 Summary
3.2 Introduction
3.3 Objective
3.4 Materials and Methods
3.4.1 Chemical reagents
3.5.1 Qualitative antennal proteome comparison of RJB workers across three time points
3.5.2 Quantitative antennal proteome comparison of RJB workers over three time points
3.6 Discussion
3.6.1 Olfactory function of adult workers requires shared antennal proteome
3.6.2 Adult worker bees evolve age—dependent antennal proteome to prime age-related biology
3.7 Conclusion
CHAPTER 4.ANTENNAL PROTEOME COMPARISON BETWEEN TWO STRAINS OF HONEYBEES REVEALS DEEP-INSIGHT INTO MOLECULAR UNDERPINNINGS OF OLFACTORY BEHAVIOUR OF WORKER BEES
4.1 Summary
4.2 Introduction
4.3 Objective
4.4.2 Antennae sampling from ITBs and RJBs
4.5 Results
4.5.1 Antennal proteome comparison between ITBs and RJBs at NEB stage
4.5.2 Antennal proteome comparison between ITBs and RJBs at NB stage
4.5.3 Antennal proteome comparison between ITBs and RJBs at FB stage
4.6 Discussion
4.6.2 ITBs and RJBs adapt divergent antennal proteome architectures during age-related polyethism
4.7 CONCLUSION
GENERAL CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
AUTHOR’S BIOGRAPHY