Hydrogen Producton Efficiency of Mixed Culturing Bacteria in theBiological Hydrogen Producing Reactor

摘要

Improving hydrogen production efficiency in the biological hydrogen-producing reactor wasone of the important issues for fermentative hydrogen production. Anaerobic sludge ofdifference period in the continuous stirred tank reactor (CSTR) was obtained. After DNAextraction of sludge, the V2 to V3 region of 16S rDNA was PCR-amplified. The diversity anddynamics of microbial communities were investigated by denaturing gradient electrophoresisgel (DGGE). The profile of DGGE showed the appearance of homogenous and heterogeneousmicrobial population in the reactor. The general bands were excised, reamplified andsequenced for phylogenetic comparisons. The results indicated that a dominant bacteriumbelongs to family Ethonalogenbacterium Harbin that has a high hydrogen production rate.Cooperative effect of microbial population further suggested that hydrogen productionefficiency was determined together by hydrogen producing bacterium and other population.