DESIGN AND FABRICATION OF MICRO/NANO-FLUIDIC CHIP PERFORMING SINGLE-CELL POSITIONING AND NANOLITER DRUG INJECTION FOR SINGLE-CELL ANALYSIS

摘要

We have developed a microfluidic chip for single-cell analysis and drug screening. The proposed device consists of surface-modified silicon channels capped with a grooved PDMS cover layer. Some region of the channel surface is treated to be hydrophobic for air leak during drug injection. We have demonstrated that the proposeded device can provide self-controlled single-cell isolation/positioning and independent drug injection onto the captured single-cell.