AIRBORN CHEMISTRY LEVITATED PROTEIN DROPLETS AS A NOVEL ANALYTICAL TOOL FOR NUCLEATION SCREENING IN MACROMOLECULAR CRYSTALLISATION

摘要

A system of novel analytical tools for fast screening of protein nucleation conditions have been developed, based on acoustic levitation and piezo-electric flow-through micro-dispensing and volume measurement in nano-volumes. The system has been used for study different proteins ability to precipitate by testing different crystallization agents. From this data precipitation diagrams are created and "normal" protein-crystallization procedure can then be performed. Described methodology consumes only minute amounts of the precious protein.