Principles practice and instrumentation for optical recording of cellular metabolism

摘要

Abstract: apter describes the principles and practice of the optical recording of cellular metabolism. Redox imaging of cells, tissues, and organs based on intrinsic fluorescent probes of cellular metabolism. Cellular metabolism may be noninvasively interrogated through the `optical method' based on the fluorescence intensity of intrinsic probe molecules (Change, 1991). The intrinsic fluorescent probes, which report on cellular metabolism, are the reduced pyridine nucleotides, NAD(P)H, and the oxidized flavoproteins. !58