THE EFFECT OF CERTAIN LABELLING PARAMETERS ON THE 99MTC – HMPAO COMPLEXFORMATION – A CHROMATOGRAPHIC STUDY

摘要

In a trial to obtain a detailed evaluation of the labelling parameters that may affect the labellingrnprocess of HMPAO complex formation, approximately fifty eight (58) technetium-99m-d,l-HMPAOrnpreparations were obtained from a commercial kit (DONG-A Pharm. Co., ITD, Seoul, Korea) with changing (6)rndifferent parameters which are: Age of sodium pertechnetate, Source of sodium pertechnetate, Order of generatorrnelution, Activity added to the kit, Specific concentration (activity per volume) and Intra-lot variability. Thernradiochemical purity of the 99mTc-HMPAO preparation could be recognized by using a combination of three thinrnlayer chromatographic (TLC) systems. Out of these six parameters, our study revealed that only the age ofrnsodium pertechnetate and the activity added to the kit were of a great influence in the %RCP of the HMPAOrncomplex, while the specific activity was noted to have a slightly significant effect.