Evaluation of Novel Carbon Nano-tube Particles in the Bacterial and Viral DNA and RNA Extraction from the Clinical Samples

摘要

Molecular techniques have become the most important methods of detecting bacterial and viral pathogens. However, current genomic extraction methods are currently limited in term of automation. In this study, carbon nano-tube was used as the vector to trap DNA and RNA molecules. The capability of carbon nano-tube to trap DNA and RNA was evaluated using samples (TB and HBV for DNA extraction and HCV for RNA extraction) with known quantities and compared to the previously established BOOM method for DNA extraction and Trizol-LS method for RNA extraction. No log difference was observed between the two methods of extraction. Carbon nano-tube is a novel material with potent for automation in molecular diagnosis.