Evaluation of the PDT Effect of Foscan~? and Fospeg~? in the LNCaP Human Prostate Cancer Cell Line

摘要

In this paper the cytotoxic effect of m-THPC, Foscan~?, as well as of the liposomal formulation of m-THPC, Fospeg~?, (kind offer of Biolitec) were studied post PDT in the human prostate cancer cell line LNCaP. The cells were incubated for 24h with 0.15 ug/ml and 1.2 ug/ml Foscan~? and Fospeg~?. Irradiation was performed with a 652nm laser and energy doses 180, 360 and 540mJ/cm2. The effect was assessed by the MTT viability test 24h after irradiation. Also the intracellular localization of Foscan~? and Fospeg~? was monitored by using Laser Scanning Confocal Microscopy Imaging. The results showed no dark toxicity either with Foscan~? or Fospeg~? at any concentration. Also irradiation at each energy dose in the absence of any photosensitizer, did not affect cellular viability. The cellular death caused after Photodynamic Treatment was dependent on m-THPC concentration and formulation, as well as the delivered energy dose. Fospeg~? was more effective as LD_(50) was achieved with 0.15μg/ml at 180mJ/cm2 while for the same cytotoxic result 1.2μg/ml Foscan~? was needed. Images from confocal microscopy revealed higher fluorescence intensity in the cytoplasm after incubation with Fospeg?, than upon incubation with Foscan~? under the same experimental conditions.