AFM Imaging of Water, Cells and Tissues

摘要

This paper presents the results of several AFM case studies of water, tumor cells, pit cells, and type- Ⅰ collagen samples. The AFM imaging procedures, surface structural characterization capabilities such as contact mode, tapping mode, and friction mode are discussed. The difference in surface morphology between the pit cell submerged in liquid and the cell in air was observed. The AFM tapping mode phase imaging technique was used to study the crosslinks in the type-Ⅰ collagen. The calibration method for accurately measuring the AFM cantilever spring constant with the help of a nanoindenter is also presented.