首页> 外文会议>Symposium on Biotechnology for Fuels and Chemicals; 20050501-04; Denver,CO(US) >Reuse of the Xylanase Enzyme in the Biobleaching Process of the Sugarcane Bagasse Acetosolv Pulp
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Reuse of the Xylanase Enzyme in the Biobleaching Process of the Sugarcane Bagasse Acetosolv Pulp

机译:木聚糖酶蔗糖浆生物漂白过程中木聚糖酶的再利用

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In this work, pretreatment-enzymatic series of the bagasse-sugarcane pulp and alkaline extraction of enzyme treated pulp were carried out. In the pretreatment an enzyme dose was utilized and acetosolv pulp suspension of 3% (w/v) with different solvents (distilled water, 0.05 mol/L acetate buffer pH 5.5 and 0.05 mol/L phosphate buffer pH 7.25) stirred at 85 rpm for 2 or 4 h. The enzymes used were pulpzyme and cartazyme, both commercial. The accompaniment of the enzymatic activity was carried out through measurement in initial and finish of each enzymatic pretreatment. The xylanase-treated pulps and xylanase-alkaline-extracted pulps were analyzed regarding kappa number and viscosity. Pulpzyme recovery was better in phosphate buffered medium (84, 46, and 23% for first, second, and third enzymatic treatment, respectively) although in aqueous medium reached only 2% for every treatments. However, the improvement of pulp properties was evidenced only in aqueous medium for pulpzyme. Cartazyme recovery was similar for both solvents (water and acetate buffer), reaching values around 19% for first enzymatic treatment and 9% for second one. Nevertheless, the pulp properties increased only in acetate buffered medium.
机译:在这项工作中,进行了蔗糖-蔗糖蔗渣纸浆的预处理-酶促系列处理和酶处理纸浆的碱提取。在预处理中,使用一定剂量的酶,并以85 rpm的转速搅拌3%(w / v)与不同溶剂(蒸馏水,0.05 mol / L乙酸盐缓冲液pH 5.5和0.05 mol / L磷酸盐缓冲液pH 7.25)的乙酰溶剂纸浆悬浮液, 2或4小时。所用的酶是纸浆酶和软骨酶,两者都是商业的。通过在每种酶预处理的开始和结束中进行测量来进行酶活性的伴随。分析了木聚糖酶处理的纸浆和木聚糖酶碱性提取的纸浆的卡伯值和粘度。磷酸盐缓冲液中的酶回收率更高(第一次,第二次和第三次酶处理分别为84%,46%和23%),尽管每次处理中在水性介质中仅达到2%。然而,仅在纸浆酶的水性介质中证明了纸浆性能的改善。两种溶剂(水和乙酸盐缓冲液)的Cartazyme回收率相似,第一次酶处理的值约为19%,第二次酶处理的值约为9%。然而,仅在乙酸盐缓冲的介质中,纸浆性能提高。

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