Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion

摘要

Confocal laser microscope (CLM) has been widely used in the fields of the non-contact surface topography, biomedical imaging, and other applications, because the confocality gives two-dimensional (2D) optical-sectioning or three-dimensional (3D) imaging capability and the stray light elimination. One potential method for scan-less 2D CLM is a combination of the line-focused CLM with the ID spectral encoding CLM. In this paper, we constructed such scan-less 2D CLM with the image acquisition time of 0.23 ms, the lateral resolution of 1.2 um, the depth resolution of 2.4 μm, and apply it for in situ imaging of plant leaf to investigate a potential for plant health monitoring.