Novel multispectral imaging microscope with applications to biomedicine

摘要

This paper describes a novel multispectral imaging microscope that can simultaneously record both spectral and spatialinformation of a sample, which can take advantage of spatial image processing and spectroscopic analysis techniques. ALiquid Crystal Tunable Filter device is used for fast wavelength selection and a cooled two-dimensional monochromeCCD for image detection. In order to acquire images that are not so dependent on imaging devices, a clever CCDexposure time control and a software based spectral and spatial calibration process is performed to diminish theinfluence of illumination, optic ununiformity, CCD’s spectral response curve and optic throughput property. A set ofmultispectral image processing and analysis software package is developed, which covers not only general imageprocessing and analysis functions, and also provides powerful analysis tools for multispectral image data, includingmultispectral image acquisition, illumination and system response calibration, spectral analysis and etc. Thecombination of spatial and spectral analysis makes it an ideal tool for the applications to biomedicine. In this paper, twoapplications in biomedicine are also presented. One is medical image segmentation. Using multispectral imagingtechniques, a mass of experiments on both marrow bone and cervical cell images showed that our segmentation resultsare highly satisfactory while with low computational cost. Another is biological imaging spectroscopic analysis in thestudy of pollen grains in rice. The results showed that the transmittance analysis of multispectral pollen images canaccurately identify the pollen abortion stage of male-sterile rice, and can easily distinguish a variety of male sterilecytoplasm.