A Novel PDMS Microfluidic Spotter for Fabrication of Protein Chips and Microarrays

摘要

A novel PDMS microfluidic spotter system has been developed for the patterning of surface microarrays that requireindividually addressing each spot area and high probe density. Microfluidic channels are used to address each spotregion and large spot arrays can be addressed in parallel. Fluorescence intensity measurement of dye-spotted samplescompared to control and pipetted drops demonstrated a minimum of a three-fold increase in dye surface density. Surfaceplasmon resonance measurement of protein-spotted samples as compared to pin-spotted samples demonstrated an 86-fold increase in protein surface density. This novel spotter system can be applied to the production of high-throughputarrays in the fields of genomics, proteomics, immunoassays and fluorescence or luminescence assays.