Resorption of Biomimetic Apatite by Osteoclasts Cultured From Bone Marrow Cells With and Without Vitamin D

摘要

FT-IR, XRD, and SEM analysis shows that the BAp coating is a nanocrystalline, nano-porous, calcium-deficient apatite containing Mg~(2+), andCO_3~(2-) ions. The bone marrow cells cultured in the presence of Vitamin D did not exhibit a greater number of differentiated osteoclasts after one week in culture. The samples without vitamin D (Figure 1a) had approximately 2 times more TRAP-positive cells than the samples with vitamin D (Figure 1b). Figure 2a is an SEM image of an osteoclast in a resorption lacuna with an extended cell body and ruffled border. After removal of adhered cells (Figure 2b), resorption pits were uncovered with the maximum dimension up to 100 μm. The resorption pits usually present circular or curved shapes on the BAp surface. The chance of finding an area (500x) containing resorption pits on the samples without vitamin D was 84% compared to a substantially lower value of 44% on the samples with vitamin D. The samples without vitamin D had 5 times more resorption pits and were larger in size than the samples without vitamin D.