A new technique for Development of perfusable multilayered blood vessel-like structures on Microfluidic Chip

摘要

A new technique has been developed for making perfusable multilayered blood vessel-like structures on a microfluidic platform. The technique can be used for making a single perfusable vessel as well as a mesh of vessels for continuous perfusion. The physical and mechanical properties of GelMa hydrogel have been optimized for formation of the blood vessel like structure. Using the newly developed technique formation of a tri-layered perfusable vessel on a microfluidic chip has been demonstrated. The multilayer vessel will have multifaceted applications including development of in vitro models of various cardiovascular diseases such as atherosclerosis and hypertension.