Stable Feeder- and Xeno-free Surfaces for Long-term Growth of Undifferentiated Human Embryonic Stem Cells

摘要

The use of iCVD to coat silicon rubber surfaces and promote embryonic stem cell adhesion is a novel approach to the goal of providing stable, xeno-free environments. iCVD coatings give stable surfaces that survived sterilization and allowed cell proliferation for more than 5 passages. The stem cells expressed pluripotency markers and a normal karyotype after propagation on this synthetic surface. The intermediate densities of protein covalently bonded to the surface gave the best results in terms of cell attachment. The definition of this synthetic approach for stem cell culture is important for future scale-up and use of stem cell culture in actual biomedical applications.