A Novel Mechanism for Targeted Delivery of Viral Vectors to Hydroxyapatite Materials

摘要

Previous work from our lab has shown the efficacy of utilizing polyglutamate domains for enhanced loading and retention of various peptides on a multitude of HA containing materials. Building on this concept, we believe this work represents a very novel application of the polyglutamate-HA mechanism for delivery. In this study we found that the addition of polyglutamate domains to both gene delivery (AAV) and protein delivery (P22) capsids resulted in greater loading on HA materials. While these studies have used model systems up to this point, we plan to use these vectors to deliver BMP-2 to enhance osteoinductivity. To our knowledge, this study is the first to use polyglutamate domains to anchor particles to HA. Furthermore, these systems serve as a proof of concept for modifying viral vectors to target HA for localized delivery of a multitude of proteins or genes.