A study on catalytic properties of the immobilized porphyrin as a mimic of cytochrome P-450

摘要

It is well known that Cytochrome P-450 5s a monooxygenase, catalyzing the following reaction, RH + NADPH + H~+ + O_2→ROH + NADP~+ + H_2O. Cytochrome P-450 is an important family of monooxygenase participating in the metabolism of many kinds of endogenous and exogenous compounds, in the last ten years, a great progress in the studies on synthesis of metalloporphyrin as a mimic of Cytochrome P-450 and the relationship between structure and function was made. The previous studies have been focused on how to increase the ability of anti-oxidation of metal porphyrin mimic and preventing porphyrin molecules from dimerization in a solution. However, as metal porphyrins lose easily their catalytic activity due to self-oxydation decomposition and are difficult to be recycled as catalysts in homo-phase systems, their usage was limited. We previously reported that the catalytic properties and stability against heat of metalloporphyrin encapsulated in supercages of molecular sieve was improved greatly. In this paper, we report the preparation and characterization of iron(Ⅲ)tetra-(p-trimethylaminophenyl) porphyrin iodide coordination compound(TAPPFe(Ⅲ)Ⅰ) immobilized on Celite and their activity of catalysis of BrO_3~- reduction.