MICROFLUIDIC TOOLS AND HIGH-CONTENT IMAGING FOR CELL THERAPY BIOPROCESSING

摘要

Culture parameters including type of matrix, feeding regimes and media formulations have to be fine-tuned in order to obtain the desired cell quality attributes that will be the basis of large scale bioprocess development. Costly and time consuming screenings, usually performed in multi-well plates, are undertaken to obtain preliminary results. In contrast, microfluidics offers the possibility to perform studies of different parameters in an integrated manner, with high-throughput analysis, lower consumption of reagents and reduction in time. This approach has the possibility to unveil valuable information in early stages of process development that can reduce the risks associated with scale-up.