Novel fluorescence methods as a base for biomedical sensoring: antioxidants, reactive radicals, NO and superoxide dynamics, immunoassay and biomembranes fluidity

摘要

We proposed and developed a series of fluorescent methods of analysis and investigation of system potentially important in biotechnology and biomedicine. Three new types of molecular probes have been used: 1) dual fluorophore-nitroxide compounds, 2) fluorescent-photochrome molecules and 3) super molecules containing fluorescent and fluorescent quenching segments. These methods utilize the following photochemical and photophysica phenomena: the fluorescence quenching, photochrome photoisomerization and energy transfer. The fluorescence properties of the new probes was intensively exploited as the basis of several methodologies which include a real time analysis of antioxidants, nitric oxide, superoxide, reactive radicals, trinitrotoluene and metal ions, investigation of molecular dynamics of biomembranes in a wide range characteristic times, detection of protein conformational transition, and characterization of surface system. Owning high sensitivity, simplicity, availability of fluorescence techniques, these methods can be widely employed. The techniques can be adapted to fibrooptic sensoring.