Advances in Phase Detection Technique of Surface Plasmon Resonance Biosensor

摘要

Surface plasmon resonance (SPR) sensing is an optical method based on evanescent wave. SPR biosensor can detect interaction of label-free biomolecules in real-time. With further development, it can become a research instrument in proteomics. SPR biosensor can be divided intensity measurement and phase measurement, and the latter possesses higher sensitivity than the former one. This paper attempts to summarize the SPR phase detection theory, discuss the major developments, compare the merits and deficiencies of various methods, and look forward to future prospects.