EFFICIENCY COMPARISON OF THREE NUCLEIC ACID EXTRACTION KITS FOR EXTRACTING BLOOD VIRUS

摘要

To compare the efficiency of different nucleic acid extraction kits for extracting blood virus. 3 commonly used kits for extraction of blood viral nucleic acid were chosen from domestic and international markets, including OMEGA kit, QIAGEN kit and WATSON kit. The nucleic acid extraction procedures were carried out according to the instructions of the 3 kits and the real-time quantitative PCR was performed for quantitative analysis. OMEGA kit had the best extraction efficiency of DNA but had the worst extraction efficiency for RNA extraction within a short time and at medium cost QIAGEN kit had good efficiency for both DNA and RNA extraction with medium term, but at the highest cost; WATSON kit had medium efficiency rate for both DNA and RNA extraction with long term extraction and at the lowest cost. For the sample with unknown capacity and category of virus, QIAGEN kit should be the first, priority and followed by WATSON kit WATSON kit should be the first choice in terms of cost.