SIGNAL AMPLIFICATION BY COUPLING INVASIVE REACTION WITH HYPERBRANCHED ROLLING CIRCLE AMPLIFICATION

摘要

Detection of specific sequences in target DNA at an ultra low level is very critical to clinical diagnosis, many approaches based on template amplification were thus developed However, these methods have a high risk of cross-contamination from amplicons, so false positive results frequently occur. Signal amplification instead of templatesequence amplification is the best way to avoid the cross-contaminatioa In this report, A novel approach was developed for signal amplification by coupling invasive reaction with hyperbranched rolling circle amplification (HRCA). The sensitivity of the proposed method is less than 1 fM artificial DNA targets; and the specificity of the method is high enough to discriminate one base difference in the target sequence. The feasibility was verified by detecting real biological samples from HBV carriers, indicating that the method has a great potencial in clinical diagnosis.