Using Electrically Tunable Lens to Improve Axial Resolution and Imaging Field in Light Sheet Fluorescence Microscope

摘要

The light sheet microscope is the primary tool for three-dimensional imaging in the life sciences. It uses a thin sheet of light to illuminate biological samples, which will reduce the luminous flux on the biological sample, thereby reducing phototoxicity and light bleaching. In addition, since only the focal plane of the detection objective is excited, the influence of the fluorophore out of focus on the contrast of the image will be reduced too.