FAST FOURIER ANALYSIS OF STRUCTURAL ORGANIZATION IN DECELLULARIZED CARTILAGE-ON-BONE LAMINATES

摘要

Denaturation of tissues can provide a unique biologicalrnenvironment for regenerative medicine application only ifrnminimal disruption of their microarchitecture is achievedrnduring the decellularization process. The goal is to keeprnthe structural integrity of such a construct as functional asrnthe tissues from which they were derived. In this work,rncartilage-on-bone laminates were decellularized throughrnenzymatic, non-ionic and ionic protocols. This workrninvestigated the effects of decellularization process on thernmicroarchitecture of cartiligous extracellular matrix;rndetermining the extent of how each process deterioratedrnthe structural organization of the network. High resolutionrnmicroscopy was used to capture cross-sectional images ofrnsamples prior to and after treatment. The variation of thernmicroarchitecture was then analysed using a well definedrnfast Fourier image processing algorithm. Statisticalrnanalysis of the results revealed how significant thernalternations among aforementioned protocols werern(p < 0.05). Ranking the treatments by their effectivenessrnin disrupting the ECM integrity, they were ordered as:rnTrypsin> SDS> Triton X-100.