The use of Hemizona Assay as a Model for Sperm Oocyte Interaction in the Evaluation of the Optimal Sperm Preperation Technique

摘要

This study was designed to evaluate the effect of different sperm preparation methods on sperm binding capacity by using the hemizona assay (HZA). Seventy men were included in the study. Four different techniques (swim-up, TEST-yolk buffer [TYB], Percoll and pentoxifylline [PTX]) were used for preparation of sperm suspensions. The routine swim-up preparation was used as the reference method. Only TYB and PTX exhibited an overall statistically significant improvement in sperm binding capacity in comparison with the swim-up preparation method (P = 0.01 and P = 0.001, respectively).