In vitro Cytotoxicity of Bacterial Cellulose Scaffold for Tissue Engineered Bone

摘要

Bacterial cellulose (BC) secreted by Acetobacter xylinum, is expected to have potential applications in bone tissue engineering. However, the degradation performance and the evaluation of the biological response of biomaterials are required to provide useful information to improve their design and application. In this article, the in vitro degradation and cytotoxicity of BC and its degradation residue were studied. Field emission scanning electron microscope (FE-SEM) was used to observe the morphology of original materials and their degradation products. The degree of degradation was valued by measuring the concentration of reducing sugar by using ultraviolet spectrophotometer. Bone-forming osteoblasts (OB) and infinite culture cell line L929 fibroblasts were used to measure the cytotocixity of materials with MTT assay. Both kinds of cells in infusion proliferate greatly in a normal form and their relative growth rate (RGR) exceeds by 75 percent, which shows the cytotoxicity of materials is graded as 0~1. Nevertheless,bone-forming OB cells, as a kind of target cells, are more susceptive on the cytotoxicity than infinite culture fibroblast cells L929. The results suggest BC without cytotoxicity is greatly promising as a kind of scaffold materials for bone tissue engineering and tissue functional cells are more suited to evaluate the cytotoxicity of biomedical materials.