We report a novel method for temperature measurement in a cesium fountain clock and experimental investigation about its dependence respect to diverse operational parameters. The temperature of the Cs atomic cloud is inferred from the width of the velocity distribution, which is obtained from two measurements in different regions of the spatial distribution of the atomic cloud using the time of flight (TOF) method. We use a simple process to determine the velocity distribution by calculating the square speed point by point from these two TOF signals. This new method is demonstrated to characterize the temperature of the Cs atoms on the CENAM Cs fountain clock. The minimum temperature reached in the CENAM Cs fountain clock is 612(193) nK.
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