Comparative Study of Hydrothermal Pretreatment for Palm Oil Empty Fruit Bunch and its Corresponding Mixture of Cellulose,Xylan,Lignin

摘要

This is a report on the behavior of lignocellulosic biomass and the equivalent model compound mixtures under hydrothermal pretreatment and its effect on enzymatic hydrolysis for glucose production. The objective is to study the effect of structural cross-link of three major components (lignin, hemicellulose, and cellulose) by comparing the actual biomass (palm oil empty fruit bunch) where the components are bound to each other, with model compounds where each compound is separated. Palm oil empty fruit bunch (EFB) was chosen as the actual biomass and the model compound of isolated cellulose, xylan and lignin were used according to the palm oil EFB lignocellulose composition. An autoclave reactor was used to conduct hydrothermal pretreatment in a temperature range 150-250 ℃ for 30 mm. To investigate the effectiveness of the pretreatment, enzymatic hydrolysis was carried out subsequently using cellulase and ss-glucosidase at 40 °C for 48 h and the glucose product was measured by HPLC. The remaining amount of cellulose was measured after hydrothermal pretreatment and enzymatic hydrolysis. The comparison between the behavior of cellulose and the efficiency of enzymatic hydrolysis under the influence of lignin and hemicellulose presence in actual biomass and model compound was elaborated in this study.