Induction of protoporphyrin biosynthesis and photodynamic inactivation of B16 melanoma cells

摘要

Abstract: The induction of protoporphyrin (PP) synthesis in B16 melanoma cells and their subsequent photodestruction was investigated. Photosensitized cells gradually disintegrated following prolonged irradiation. The photosensitized cells showed an immediate potassium loss compensated only partially by influx of Na and other ions. The dominant characteristics of these cells were potassium efflux, water influx and ultrastructural damage in subcellular compartments. In conclusion, a highly efficient system for killing B16 melanoma cells was achieved by photosensitization of endogenous PP produced subsequent to chemical induction. !13