Measuring cytochrome c redox state using Resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light

摘要

Photobiomodulation, also known as low level laser therapy (LLLT), is a technique that uses light in the red and nearinfrared (NIR) range (600-900 nm) to elicit a clinically beneficial physiological change in tissue. This physiological changeis thought to begin in the mitochondria by altering the metabolic rate for the electron transport chain (ETC). ResonanceRaman spectroscopy at 532 nm was used to determine the reduction/oxidation (redox) state of cytochrome c in isolatedmitochondria after undergoing LLLT. Mitochondria from hTERT-RPE1 cells were isolated and placed in glutamate bufferand then exposed to violet (405 nm) or red (635 nm) light. The resonance Raman spectrum of the cytochrome c redox statebefore and after light illumination was measured. This gives us an insight into the types of metabolic changes that occurswithin the mitochondria while being illuminated by light during photobiomodulation.